CRABP1 Antibody (C-1)

Immunocytochemistry/Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in NIH-3T3 Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: CRABP1 Antibody (C-1) [NB300-539] - Both normal and cancer biopsies of deparaffinized Human thyroid tissues.

Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in Hela cells (green) compared to an isotype control (blue).

Immunocytochemistry/Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in Hela Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI at a dilution of 1:200 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunocytochemistry/Immunofluorescence: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI using Anti-CRABPI Monoclonal Antibody (C-1) shows staining in MCF-7 Cells. CRABPI staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing CRABPI at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.

Immunohistochemistry-Paraffin: CRABP1 Antibody (C-1) [NB300-539] - Both normal and cancer biopsies of deparaffinized Human breast carcinoma tissues.

Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in NIH-3T3 cells (green) compared to an isotype control (blue).

Flow Cytometry: CRABP1 Antibody (C-1) [NB300-539] - Analysis of CRABPI in MCF-7 cells (green) compared to an isotype control (blue).

Product Discontinued

CRABP1 Antibody (C-1) Summary

• Immunogen: Oxidized CRABPI from bovine retina.
• Specificity: Detects cellular Retinoic Acid Binding Protein 1 (CRABP 1). This does not cross-react with Cellular Retinol Binding Protein (CRBP), performic acid oxidized CRBP, interphotoreceptor retinoid binding protein or retinol binding protein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRABP1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1 - 2 ug
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:200
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Frozen 1:250
  • Immunohistochemistry-Paraffin 1:1000
  • Western Blot 1:1000
• Application Notes: WB: Detects an approx. 16 kDa protein representing CRABP 1 from rat retinal supernatant. IHC: Staining of CRABP 1 in rat retina results in its localization to amacrine somata and laminae in the inner plexiform layer.

Reactivity Notes

Cross-reactivity with Reptiles.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Concentration: This product is unpurified. The exact concentration of antibody is not quantifiable.
• Purity: Unpurified

Alternate Names for CRABP1 Antibody (C-1)

• cellular retinoic acid binding protein 1
• CRABP
• CRABPI
• CRABP-Icellular retinoic acid-binding protein 1
• RBP5Cellular retinoic acid-binding protein I

Background

Retinoic acid (RA), a metabolite of vitamin A, plays an important role in growth and differentiation by modulation of expression of certain genes. RA functions through its interaction with the nuclear protein, retinoic acid receptor (RAR) and the 9-cis RA with retinoid X receptor (RXR). The Cellular Retinoic Acid Binding Proteins (CRABP) bind RA with a high affinity and belong to the family of fatty acid binding proteins. Two forms of CRABP have been characterized, CRABP 1 and CRABP 2 which share ~75% sequence conservation. The main role of CRABP 1 may be to protect retinoids from non-specific dehydrogenases and direct them to specific enzymes. The precise role of CRABP 2 is still poorly understood but it seems to play a role in the interaction of ligands (RA, 9-cis RA) with nuclear receptors (RAR/RXR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CRABP1 Antibody (NB300-539)(6)

Publications using NB300-539

• Zeng B, Liu Z, Lu Y et al. The single-cell and spatial transcriptional landscape of human gastrulation and early brain development Cell stem cell 2023-05-12 [PMID: 37192616] (IHC, Human)
• Kelley MW et al. The developing organ of Corti contains retinoic acid forms supernumerary hair cells in response to exogenous retinoic acid in culture. Development 119:1041-53. 1993-01-01 [PMID: 8306874]
• Kelley MW et al. Retinoic acid promotes differentiation of photoreceptors in vitro. Development 120:2091-102. 1994-01-01 [PMID: 7925013]
• Gaur VP et al. Localization of cellular retinoic acid-binding protein to amacrine cells of rat retina. Exp Eye Res 50:505-11. 1990-01-01 [PMID: 2164945]
• De Leeuw AM et al. Immunolocalization of cellular retinol-, retinaldehyde- retinoic acid-binding proteins in rat retina during pre- postnatal development. J Neurocytol 19:253-64. 1990-01-01 [PMID: 2162910]
• Milam AH et al. Immunolocalization of cellular retinoic acid binding protein to Muller cells /or a subpopulation of GABA-positive amacrine cells in retinas of different species. J Comp Neurol 296:123-9. 1990-01-01 [PMID: 2162866]

Bioinformatics

• Gene Symbol: CRABP1
• Entrez:
  • 1381(Human)
  • 25061(Rat)